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Composite

Part:BBa_K088002:Design

Designed by: Hai Chen   Group: iGEM08_CPU-NanJing   (2008-10-13)


pLac+RBS+lacI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1372
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows BBa_C0012 to be used as a inverter or as a detector of lactose or IPTG.


Source

The pLac regulatory region is a 243 base-pair sequence with two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor BBa_C0012,which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows.

References